Midwifery and Women’s Health

Biography

Biography: Fatemeh Baghbani

Abstract

Statement of the Problem: Development of multidrug resistance (MDR) mediated by overexpression of P-glycoproteins is one of the major obstacles to effective cancer chemotherapy. Ultrasound-responsive perfluorocarbon nanoemulsions are a class of new multifunctional smart nanocarriers which combine diagnostic properties with therapeutic properties and release their drug payload in a controlled manner in response to ultrasound. Therefore, combination therapy using chemoterapeutic and chemosensitizing agents co-entrapped in these nanocarriers seems beneficial for bypassing MDR in overian cancer. 

Methodology &Theoretical Orientation: In the present study, multifunctional smart alginate/perfluorohexane nanodroplets were developed for co-delivery of doxorubicin and curcumin (a strong chemosensitizer). Findings: The nanodroplets with the average particle size of 55.1 nm were obtained via nanoemulsion process. The entrapment efficiency of doxorubicin was 92.3%. Ultrasound-mediated drug release kinetic was evaluated at two different frequencies of 28 kHz (low frequency) and 1 MHz (high frequency). Low frequency ultrasound resulted in higher triggered drug release from nanodroplets. The nanodroplets showed strong ultrasound contrast via droplet to bubble transition as confirmed via B-mode ultrasound imaging. Enhanced cytotoxicity in adriamycin-resistant A2780 ovarian cancer cells was observed for doxorubicin-curcumin loaded nanodroplets (Dox-Cur-NDs) compared to doxorubicin loaded nanodroplets (Dox-NDs) because of the synergistic effects of doxorubicin and curcumin.  However, ultrasound irradiation significantly increased the cytotoxicity of Dox-Cur-NDs. Finally, in vivo ovarian cancer treatment using Dox-Cur-NDs combined with ultrasound irradiation resulted in efficient tumor regression. According to the present study, nanotherapy of multidrug resistant human ovarian cancer using ultrasound responsive doxorubicin-curcumin co-loaded alginate-shelled nanodroplets combined with ultrasound irradiation could be a promising modality for the future of cancer treatment